and characterization of protein aggregates by
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Detection and characterization of protein aggregates by ...
2007-2-1 Detection of protein aggregates is needed in the process of protein characterization and during optimization of pharmaceutical formulations. This paper describes a technique, which consists of analysing protein aggregates by fluorescence microscopy after staining with the hydrophobic probe Nile Red.
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(PDF) Detection and characterization of protein aggregates ...
Detection of protein aggregates is needed in the. process of protein characterization and during optimization of pharmaceutical formulations. This paper describes a technique, which consists of ...
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Separation and characterization of protein aggregates and ...
Field flow fractionation (FFF) is a technique that holds great promise for the analysis and characterization of protein aggregates and particles, due to its wide dynamic range and matrix-free separation mechanism. FFF can be routinely used to achieve good monomer-oligomer separation and quantificati
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Rapid Detection and Characterization of Protein
2018-7-19 aggregates during manufacturing, shipping and storage (1-4). In addition to loss of functional protein, aggregates may induce unwanted immune responses once administered to patients (5-7). Because of this, monitoring the type and amount of aggregate in a protein therapeutic has become an important concern for
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Separation and Characterization of Protein Aggregates and ...
Request PDF Separation and Characterization of Protein Aggregates and Particles by Field Flow Fractionation Field flow fractionation (FFF) is a technique that holds great promise for the ...
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Characterization of protein aggregation: The case of a ...
2007-1-1 “Loose” protein aggregates formed by non-covalent bonds are therefore more difficult to detect and quantify. The size of aggregates can be determined by static or dynamic light scattering; however in the case of a multimodal distribution (e.g. presence of monomers and aggregates), the results are often biased towards the larger species.
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Fluorogenic Detection and Characterization of Proteins by ...
Protein is one of the four most important biomacromolecules in living systems. The detection, quantification, localization, and characterization of proteins is essential for an understanding of biological fundamentals, as well as for the diagnostics
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Holographic Characterization of Protein Aggregates
Holographic characterization directly measures the radius and refractive index of subvisible protein aggregates and offers insights into their morphologies. The measurement proceeds fast enough to build up population averages for time-resolved studies and lends itself to tracking trends in protein aggregation arising from changing environmental ...
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Holographic Characterization of Protein Aggregates ...
2016-3-1 Holographic characterization originally was developed for analyzing spherical particles.11, 19 Rigorously generalizing the analysis to account for the detailed structure of aspherical and inhomogeneous objects is prohibitively slow because of the analytical complexity and the associated computational burden.20, 21, 22 We, therefore, use the idealized spherical
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(PDF) Holographic Characterization of Protein Aggregates
Holographic characterization measurements are compared with results obtained with microflow imaging and dynamic light scattering. Protein aggregates flowing down a microfluidic channel form ...
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(PDF) Detection and characterization of protein aggregates ...
Detection of protein aggregates is needed in the. process of protein characterization and during optimization of pharmaceutical formulations. This paper describes a technique, which consists of ...
Read More
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Detection and characterization of protein aggregates by ...
Please cite this article as: Barthelemy Demeule et al., Detection and characterization of protein aggregates by fluorescence microscopy,´ International Journal of Pharmaceutics (2006), doi:10 ...
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Separation and Characterization of Protein Aggregates and ...
Request PDF Separation and Characterization of Protein Aggregates and Particles by Field Flow Fractionation Field flow fractionation (FFF) is a technique that holds great promise for the ...
Read More
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Rapid Detection and Characterization of Protein
2018-7-19 aggregates during manufacturing, shipping and storage (1-4). In addition to loss of functional protein, aggregates may induce unwanted immune responses once administered to patients (5-7). Because of this, monitoring the type and amount of aggregate in a protein therapeutic has become an important concern for
Read More
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Separation and Characterization of Protein Aggregates and ...
2021-2-15 Keywords:Protein, aggregate, oligomer, particle, field flow fractionation. Abstract: Field flow fractionation (FFF) is a technique that holds great promise for the analysis and characterization of protein aggregates and particles, due to its wide dynamic range and matrix-free separation mechanism. FFF can be routinely used to achieve good ...
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In situ characterization of protein aggregates in human ...
2016-7-4 In situ characterization of protein aggregates in human tissues affected by light chain amyloidosis: a FTIR microspectroscopy study. Sci. Rep. 6 , 29096; doi: 10.1038/srep29096 (2016).
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(PDF) Holographic Characterization of Protein Aggregates
Holographic characterization measurements are compared with results obtained with microflow imaging and dynamic light scattering. Protein aggregates flowing down a microfluidic channel form ...
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Holographic Characterization of Protein Aggregates in
oil contamination in protein formulations.5 Most conventional particle characterization technologies cannot distinguish quanti-tatively between silicone oil and protein aggregates for sizes less than 5 mm, making it difficult to unambiguously confirm the presence of
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Characterization of aggregate/aggresome structures
2015-10-6 Detection of protein aggregates/aggresomes during BmNPV infection. ProteoStat Aggresome Detection Kit has been used extensively to specifically detect denatured and/or misfolded protein aggregates ...
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Strategies for the Assessment of Protein Aggregates in ...
2010-10-23 Extended characterization data, if available, may then help to establish a relationship between the presence and nature of aggregates in a protein pharmaceutical on the one hand and immunogenicity data emerging from use of the product in humans on the other.
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Detection and characterization of protein aggregates by ...
Please cite this article as: Barthelemy Demeule et al., Detection and characterization of protein aggregates by fluorescence microscopy,´ International Journal of Pharmaceutics (2006), doi:10 ...
Read More
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Separation and Characterization of Protein Aggregates and ...
2020-11-24 Keywords:Protein, aggregate, oligomer, particle, field flow fractionation. Abstract: Field flow fractionation (FFF) is a technique that holds great promise for the analysis and characterization of protein aggregates and particles, due to its wide dynamic range and matrix-free separation mechanism. FFF can be routinely used to achieve good ...
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Methods for Characterization of Protein Aggregates ...
2014-11-16 Physicochemical characterization of protein aggregates is important on one hand, due to its large impact in understanding many diseases for which formation of protein aggregates is one of the pathological hallmarks. On the other hand, recently it has been observed that bacterial inclusion bodies (IBs) are also highly pure proteinaceous ...
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(PDF) Formation and characterization of chitosan-protein ...
Characterization of chitosan and protein aggregates by light scattering The chito san sample was characterized in aqueous solution by measuring M a , R ga and R ha
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In situ characterization of protein aggregates in human ...
2016-7-4 In situ characterization of protein aggregates in human tissues affected by light chain amyloidosis: a FTIR microspectroscopy study. Sci. Rep. 6 ,
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Holographic characterization of protein aggregates
2018-8-28 Holographic characterization of protein aggregates Chen Wang,1 Xiao Zhong,2 David B. Ru ner,3 Alexandra Stutt,4 Laura Philips,3 Michael D. Ward,2 and David G. Grier1 1Department of Physics and Center for Soft Matter Research, New York University, New York, NY 10003 2Department of Chemistry and Molecular Design Institute, New York University, New
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Characterization of aggregate load and pattern in living ...
2018-3-16 Protein aggregation is both a hallmark of and a driving force for a number of diseases. It is therefore important to identify the nature of these aggregates and the mechanism(s) by which the cell counteracts their detrimental properties. Currently, the study of aggregation in vivo is performed primarily using fluorescently tagged versions of proteins and analyzing the
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Characterization of aggregate/aggresome structures
2015-10-6 Detection of protein aggregates/aggresomes during BmNPV infection. ProteoStat Aggresome Detection Kit has been used extensively to specifically detect denatured and/or misfolded protein aggregates ...
Read More
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Hydrodynamic size-based separation and characterization
2014-12-18 Tanase, M., Zolla, V., Clement, C. et al. Hydrodynamic size-based separation and characterization of protein aggregates from total cell lysates. Nat Protoc 10, 134–148 (2015 ...
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Higher-Order Structure and Protein Aggregate ...
2013-2-1 Filipe V, Poole R, Kutscher M, Forier K, Braeckmans K, Jiskoot W (2011) Fluorescence single particle tracking for the characterization of submicron protein aggregates in biological fluids and complex formulations. Pharm Res
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